| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mayo, Phoebe K (12)
|
VASEVA |
31.40 |
30.46 |
-0.94 |
| 2 |
Fass, Cana O (12)
|
VAWAC |
31.92 |
31.34 |
-0.58 |
| 3 |
Kohler, Sofia A (12)
|
VATAC |
31.25 |
31.51 |
0.26 |
| 4 |
Musto, Bernadette I (12)
|
VATAC |
32.01 |
31.62 |
-0.39 |
| 5 |
Spruill, Skylar M (12)
|
VASEVA |
35.99 |
32.74 |
-3.25 |
| 6 |
Creelman, Stephanie E (12)
|
VASEVA |
34.67 |
32.91 |
-1.76 |
| 7 |
Daugherty, Samantha E (12)
|
VASEVA |
33.60 |
32.94 |
-0.66 |
| 8 |
O'Neil, Alexis A (12)
|
VAWAC |
33.73 |
35.46 |
1.73 |
| 9 |
Drozda, Isabella L (12)
|
VASEVA |
37.70 |
36.71 |
-0.99 |
| 10 |
Arbuckle, Ella V (12)
|
VATAC |
37.42 |
36.79 |
-0.63 |
| 11 |
Lee, Karen Y (12)
|
VASEVA |
39.68 |
36.86 |
-2.82 |
| 12 |
Avery, Anne S (12)
|
VATAC |
35.94 |
37.35 |
1.41 |
| 13 |
Balentine, Camryn A (12)
|
VATAC |
38.88 |
37.60 |
-1.28 |
| 14 |
Holcomb, Kathyrn J (12)
|
VASEVA |
38.83 |
38.36 |
-0.47 |
| 15 |
Schulz, Audrey M (12)
|
VAECAT |
|
43.82 |
- |